Peptide Library

Peptide libraries, which contain a number of peptides consisting of a series of amino acids, are a new approach to the study of protein structure and function. They provide a rapid and cost-effective solution to identify key bioactive peptides for a wide range of bioactivity screening purposes and have widely used in biological research. Peptide library has become a powerful tool in biochemistry and drug applications. Due to the growing interest in peptide drugs, the demand for peptide libraries from pharmaceutical companies continues to grow.

With over 25 years of experience in the design and production of custom peptide libraries, BOC Sciences, a team of professionals, is committed to providing high-quality peptide library services at a very competitive prices using our accumulated knowledge and experience in synthetic chemistry.

Figure 1. Schematic presentation of constructing the CX7C-peptide library. (Baalen, A. V., Stephani, U. 2002)

Application

• Screening of receptors and ligands
• Drug discovery
• Protein interactions
• Proteome function
• Peptide nucleic acids
• Screening for enzyme substrates or peptidase inhibitors
• Antigen binding site or antigen epitope mapping
• Discovery of T-cell epitopes
• Antibody binding sites on antigens (proteins)
• Cross-reactivity of antibodies with related proteins
• Identification of short peptide antigens

Peptide Library Design

• Surpport different peptide library size, format and synthesis scale
• BOC Sciences has employed advanced CLIPS technology to construct unique conformational peptide libraries
• Various peptide modifications are available depending on specific requirement
• Fast and reliable custom synthesis with global delivery

Figure 2. Illustration of the on-chip screening for peptide-based protein binders. (Schwaar, T.; et al. 2019)

Peptide Library Design Options

BOC Sciences provides advice on creating the most efficient and cost-effective library design, offering a range of library design options:

Random Library

We apply 20 natural amino acids to randomly and simultaneously substitute the selected residues of a peptide sequences using a ‘shotgun approach’ to create our random peptide libraries.

Overlapping Peptide Library

Our experienced experts divide the original protein or peptide sequence into many overlapping peptides of the same length to generate an overlapping peptide libraries. We fully consider peptide length and peptide offset that play important role in the library construction.

Alanine Scanning Peptide Library

In order to build an alanine scanning peptide library, we use alanine to substitute the residues at each amino acid position of the original peptide, which can generate alanine with less or no binding or activity.

Positional Scanning Library

We use natural amino acids to replace one or more selected residues within a peptide sequence. The amino acids we apply can increase activity or binding and can be identified using a positional scanning library.

Truncation Peptide Library

Aiming find the minimum length of an epitope, BOC Sciences can deliver a scrambled library by reducing the peptide length of amino acid from each terminus creating a set of truncated peptides with different lengths.

Scrambled Library

At BOC Sciences, we create a reliable scrambled library via permutation of the original peptide sequence and represents all alternative peptides with the same amino acid composition.

T-cell Truncated Peptide Library

Our well-developed T-cell truncated peptide library is composed of truncated peptide combinations to ensure that the entire T-cell epitope can be spanned across structural domains

Micro-scale Peptide library

We establish a micro-scale peptide library that is composed of multiple peptides containing systematic combinations of amino acids to offer peptides at micromolar scales. Our unique library is an optimal choice for targeted proteomics applications and preliminary screening.

Peptide Cyclization Library

BOC Sciences supports a variety of cyclization strategies to synthesize peptides such as head-to-tail cyclization, side-chain-to-side-chain, head-to-side-chain, and side-chain-to-tail cyclization.

Peptide Pool Service

As a complement to our peptide library service, we have developed multiple pools of peptides for the accurate stimulation of T-cells. We can prepare customized peptide pools according to your customized specifications with pre-pooling LC-MS validation, and post-pooling marker validation QC options.

Peptide HTS library

BOC Sciences has introduced an advanced peptide HTS library synthesis platform, to synthesize high throughput peptides with industry leading speed and flexibility.

Custom Library Synthesis and Delivery

• Custom peptide arrays are available in various sizes and formats, ranging from a small set of peptides to libraries of 1,000 different species.
• Peptide lengths are typically ranging from 5 to 30 amino acids, which is sufficient for most applications
• For the special requirement of conformational constraints or post-translational modifications, we also provide peptide libraries of up to 50 amino acid
• Peptide libraries are generally manufactured at 4 μmol scale, yielding 1 to 4 mg of crude peptide, ideal for fast and efficient screening campaigns
• All purified peptides are accompanied by a complete QC data set, including RP-HPLC, MS and COA reports to ensure high quality
• Standard delivery times of around 3-4 weeks
• Delivered as freeze-dried peptides in 96-well plates or individual microtubes
• All peptides in the library will be delivered as TFA-salts as lyophilized powder
• BOC Sciences also produces peptide libraries with different structural modifications

BOC Sciences provides professional, rapid and high-quality services of Peptide Library design at competitive prices for global customers. Personalized and customized services of Peptide Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!