Peptide pools are a group of immunostimulatory epitopes in T-cell stimulation of HIV, HCV, influenza and other infectious diseases. They are a collection of small peptides of specific length and different sequences. Nowadays, peptide pools are powerful tools for drug design, protein interactions, biochemistry and drug applications.
BOC Sciences peptide pools service can provide customized sequences and pooling protocols to meet the requirements of different projects. In addition to quality-control reports such as pre-pooling LC-MS validation and post-pooling marker validation QC options. Post-pooling marker peptides have unique, distinguishable properties and can be used to verify that all desired peptides have been added to the given peptide pool system.
Application
- Drug screening
- Target identification
- Antigen epitope mapping
- Vaccine development
Figure 1. Randomized peptide pools as a technique to evaluate TCR repertoire from PBMCs or sorted cell subsets. (Barber, J. S.; et al. 2013)
Peptide Pooling Service platform
We use pooled peptides, such as overlapping peptides from an antigen or proteotypic peptides, to save the time of testing many peptides in parallel. BOC Sciences Peptide Pooling Service platform offers a wide range of peptide pools of different sizes which have been optimized for different applications:
- Peptide Pools
- Antigen Discovery Peptide Pools
- Peptide Pools for Immunopeptidomics
- Peptide Pools for Targeted Proteomics
We have designed our peptide pools based on the standard for stimulating antigen-specific T cells in functional T-cell assays. BOC Sciences offers a wide variety of peptide pools for different TAAs and viral, bacterial or fungal antigens
Our antigen discovery pool is an excellent choice for antigen target discovery, T-cell epitope discovery and biological screening. All the peptides in the antigen discovery pool are synthesized fully automatically in small quantities and have been purified by dialysis. BOC Sciences is able to deliver the antigen discovery pool very quickly and at low cost
Our peptide pools for immunopeptidomics are synthesized and optimized for immunopeptidomics which can provide fast and inexpensive access to reference peptides to improve the accuracy of neo-epitope identification
Peptide pools for targeted proteomics are stable isotope-labeled peptide libraries that are commonly used for relative protein quantification by mass spectrometry-based assays. BOC Sciences offers a variety of peptide libraries for TAAs, cytokines or organelles
What we Deliver?
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- LC-MS on marker peptides (up to 8) in final pool to guarantee presence of all peptides within the final pool
- Standard delivery times of around 3-4 weeks depending on different pool size
- Delivered as 96-well plate or 100-tube rack
- COA, MS, and HPLC validation data for each peptide before pooling are provided
BOC Sciences provides professional, rapid and high-quality services of Peptide Pool Service at competitive prices for global customers. Personalized and customized services of Peptide Pool Service can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Barber, J. S.; et al. Peptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases. Proceedings of the National Academy of Sciences of the United States of America. 2013. 110(20): 8164-8169.
