T-cell epitopes are kind of short peptide sequences that range in length from 8-11 amino acids on the MHC I complex to 15-24 amino acids on the MHC II complex. T-cell related antigen peptides targeting proteins can be used to screen out the optimal T-cell epitopes. Peptide libraries have proven to be useful for identifying short peptide sequences within larger epitopes because they enable to span hundreds of peptide combinations. Scientists pool these truncated peptide fragments together and deconvolute the identified positive cell pool to determine the T-cell epitope sequences.
BOC Sciences T-cell truncated peptide library is composed of truncated peptide combinations to ensure that the entire T-cell epitope is spanned across structural domains.
Figure 1. Overview of T-cell library methodology. (Theaker, S. M.; et al. 2016)
Library Design
- Firstly, the protein sequence of interest is selected, and T-cell related antigenic peptides are designed for the target proteins
- Then, we select the longest peptide sequence in the pool. The peptides are then gradually truncated into short peptides from both ends to form a peptide library
- Finally, the obtained peptide library is mixed into a peptide pool of different length of peptides that span the entire sequence length to screen for the best T-cell epitopes
Features of Our Library
- Our truncated peptide library allows the testing of all possible T-cell epitopes across a protein of interest
- In each test tube, we provide an equimolar mixture of four C-terminal peptides for each nominal 11-mer
- Positive tubes can be deconvoluted for the subsequent epitope identification after screening
What we Deliver?
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
- Our expert teams can provide peptide library containing up to 50 amino acid if there is special demand
- Standard delivery times of around 3-4 weeks depending on different library size and peptide length
- Delivered as freeze-dried in 96-well plates for subsequent experiments
- MS and HPLC analysis results are provided for each peptide product
- All peptides in the library can be de-TFA salt treatment, also can be converted to acetate according to the customer's requirements
BOC Sciences provides professional, rapid and high-quality services of T-cell Truncated Peptide Library design at competitive prices for global customers. Personalized and customized services of T-cell Truncated Peptide Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Theaker, S. M.; et al. T-cell libraries allow simple parallel generation of multiple peptide-specific human T-cell clones. Journal of immunological methods. 2016. 430: 43-50.
