Multiplex Combinatorial Biosynthesis (MCB)

Natural products (NPs) have always played key roles in drug discovery. Scientific experts now are expanding the capabilities of natural chemistry through combinatorial biosynthesis, for example different biosynthetic enzymes are modified to alter functional groups, regiochemistry and scaffold, etc. Diversity-oriented synthesis (DOS) is widely used to build both quantitative and qualitative libraries of natural product. Emerging synthetic biology technologies can further accelerate the refinement of combinatorial biosynthesis as a incredible platform to diversify natural chemical drug leads. MCB technology has therefore been developed to greatly expand the diversity and application of natural molecules. In addition, with the deep understanding of the biosynthetic mechanism, the application of this technology has huge potential in building libraries of polyketide, polypeptide and polyketide-polypeptide hybrid natural products.

BOC Sciences has offered new solutions for the combinatorial biosynthesis of natural products using MCB approaches, which can accelerate novel drug discovery by combining synthetic biology with synthetic chemistry.

Figure 1. Multiplex combinatorial biosynthesis process. (Yan, Y.; et al. 2013)

Our Strategies

BOC Sciences combines MCB techniques and DOS strategies to apply the concepts of combinatorial chemistry to all stages of biosynthesis:

• We first identify the determinants of structural changes in the biosynthesis of natural products
• Then, a combination of in vivo mutational biosynthesis, pathway modifications and in vitro chemistry-catalyzed biotransformation is applied
• Finally, MCB method is applied for the preparation of starting units, selection of extended substitutions and post-modifications of scaffolds in biosynthesis
• Ultimately, hundreds of natural products with the same scaffold are obtained successfully

Features of MCB

• The active molecules obtained from the screening can be used for subsequent structural validation, activity validation or other testings
• Generate the natural product library with both quantity and quality
• Keep pace with high-throughput screening technologies to meet the demand of structural diversity

BOC Sciences provides professional, rapid and high-quality services of Multiplex Combinatorial Biosynthesis (MCB) at competitive prices for global customers. Personalized and customized services of Multiplex Combinatorial Biosynthesis (MCB) can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!