The purpose of building truncation peptide libraries is to determine the shortest amino acid sequence required for maintaing activity in a peptide. Experts systematically truncate the peptide sequence, that is, remove the flanking residues of the original peptide. To construct this unique library. Generally, an Alanine Scanning is employed to determine the essential amino acids in the peptide, and the truncation direction can then be determined and adjusted to maintain these residues while truncating the opposite end.
With our advanced mutagenic library construction platform, BOC Sciences provides high quality truncation library design and synthesis services to meet the requirements of different projects.
Applications
Incremental truncation technology has multiple applications in protein engineering:
- Detection of regions responsible for protein-protein interactions
- Study the connection between sequence, structure and function
- Identify protein fragments that can non-covalently combine to form functional proteins through stochastic pairing of fragments in libraries
- Generate integrated fusion libraries
Figure 1. Epitope mapping using truncation peptide arrays. (Oyen, D.; et al. 2017)
Library Design
BOC Sciences employs advanced incremental truncation technology to generate peptide libraries when each onebase-pair in a gene or gene fragment is missing, or detailed structural information is unavailable. Our experts have developed several useful strategies:
- Combination of α-phosphothioate dNTPs by PCR
- Time-dependent nuclease method
- Primer extensions (THIO(pcr) truncation and THIO(extension) truncation, respectively)
Custom Library Synthesis and Delivery
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
- Our expert teams can provide peptide library containing up to 50 amino acid if there is special demand
- Standard delivery times of around 3-4 weeks depending on different library size and peptide length
- Delivered as freeze-dried in 96-well plates for subsequent experiments
- MS and HPLC analysis results are provided for each peptide product
- All peptides in the library can be de-TFA salt treatment, also can be converted to acetate according to the customer's requirements
BOC Sciences provides professional, rapid and high-quality services of Truncation Peptide Library design at competitive prices for global customers. Personalized and customized services of Truncation Peptide Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Oyen, D.; et al. Structural basis for antibody recognition of the NANP repeats in Plasmodium falciparum circumsporozoite protein. Proceedings of the National Academy of Sciences of the United States of America. 2017: E10438-E10445.
