In order to study the cancer development and progression, scientists have made various strategies to determine the preferred substrate cleavage sequence of proteases since sequence plays a crucial role in the identification of endogenous protease substrates and signaling pathways. Various researchers have demonstrated multiple techniques to address this problem, but most of them can be very time-consuming. Position scanning is an important tool for peptide sequence optimization, where a selected region or site of a peptide is systematically replaced with other amino acids. Therefore, positional scanning library is designed and synthesized to help researchers discover specific regions that have a particular impact or activity at a particular location.
Aiming to screen out proteases and find their preferred residues at positions of interest, BOC Sciences has implemented diverse positional scanning synthetic libraries. In addition, these sequences can be rapidly evaluated by subsequent kinetic study on single peptide substrates.
Applications
- Increase the activity of enzyme substrates
- Enhance antibody epitopes
- Improve T-cell epitopes
- Optimize peptide binding sites
Figure 1. Positional scanning method. (Wavreille, A. 2006)
Library Design
- Positional Scanning Library
- Combinatorial 2-Positional Scanning Library
- Combinatorial 3-Positional Scanning Library
We have designed a Positional Scanning Library that identifies the target amino acid at a single position and replaces it with all other natural amino acids at once. Then, our experts can identify the preferred amino acid residues at that position through the increased activity.
Our combinatorial 2-Positional Scanning Library is used to identify target amino acids at two given positions and replaces them with all other natural amino acids at once.
BOC Sciences can identify amino acids at three given positions and replaces them with other natural amino acids at once.
Our strategy
- We generate a positional scanning library by replacing one or more selected residues at the target site within a peptide sequence with all other natural (or non-natural) amino acids.
- In the combinatorial positional scanning library, two or more residues at different positions in each peptide are varied and our professional team considers the effect of multiple positions on the biological activity of the peptide sequence.
- BOC Sciences utilizes the positional scanning library to identify amino acids that increase activity or binding, and to design sequences with improved properties.
Custom Library Synthesis and Delivery
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
- Our expert teams can provide peptide library containing up to 50 amino acid if there is special demand
- Standard delivery times of around 3-4 weeks depending on different library size and peptide length
- Delivered as freeze-dried in 96-well plates for subsequent experiments
- MS and HPLC analysis results are provided for each peptide product
- All peptides in the library can be de-TFA salt treatment, also can be converted to acetate according to the customer's requirements
BOC Sciences provides professional, rapid and high-quality services of Positional Scanning Library design at competitive prices for global customers. Personalized and customized services of Positional Scanning Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Wavreille, A. SRC homology 2 domain proteins binding specificity: from combinatorial chemistry to cell-permeable inhibitors. 2006.
