Peptide Pools for Targeted Proteomics

Targeted proteomics enables targeted analysis of specific peptides in complex samples, which can determine the presence or absence of a peptide or protein in the sample (provided that the signal is higher than the detection limit), and quantify the peptides or proteins in different samples for expression difference comparison. Targeted proteomics can be seen as a new generation of mass spectrometry-based Western Blot technology, but it is more accurate than traditional WB method and does not require antibodies. In general, scientists perform analysis based on Orbitrap mass spectrometry.

The collection target of this method is the peptide obtained by enzymatic digestion of the target protein, so it is important to select the target peptide and the appropriate collection approach. BOC Sciences is committed to providing our technical support to deliver a high quality peptide pool for targeted proteomics.

Application

• Development of mass spectrometry-based proteomics assays
• Relative protein quantification
• Development of kits to quantify entire proteomic pathways
• Clinical monitoring of therapeutic interventions and disease states
• Mass spectrometry-based assays (SISCAPA, MRN, etc.)

Figure 1. Schematics of MS-based proteomics and data-acquisition strategies. (Kosaka, P. M.; et al. 2018)

Our Services

• Our peptide pools for targeted proteomics are suitable for targeted proteomics studies, such as protein detection and protein quantification by mass spectrometry
• BOC Sciences supports the construction of a library of proteotypic stable-isotope-labeled peptides such as SIL peptide pools
• Our peptide pool services platforms support various modification operations such as alkylation, PTMs (phosphorylation, glycosylation, methylation, etc.)
• All SIL peptides of our peptide pools contain heavy arginine or heavy lysine
• We offer additional services to quantify multiple proteins in a single sample
• Our peptide pools can be used for multiple analyses of disease states and treatment success

What we Deliver?

• At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
• LC-MS on marker peptides (up to 8) in final pool to guarantee presence of all peptides within the final pool
• Standard delivery times of around 3-4 weeks depending on different pool size
• Delivered as 96-well plate or 100-tube rack
• COA, MS, and HPLC validation data for each peptide before pooling are provided

BOC Sciences provides professional, rapid and high-quality services of Peptide Pools for Targeted Proteomics design at competitive prices for global customers. Personalized and customized services of Peptide Pools for Targeted Proteomics design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!