Peptide Cyclization Library
Cyclization modifications can confer several advantages to peptides that cumulatively make them more drug-like. Constraining peptides via cyclization is a common strategy to mimic (protein) secondary structures (e.g. peptide rings) or to create peptides with enhanced conformational stability (compared to their linear analogs).
Aiming to construct a top-quality -peptide cyclization library, BOC Sciences has established several reaction platforms to create various types of conformationally-constrained peptides with robust reactions and high yields.
Application
- Discovery of peptides with desirable stability
- Mimic secondary protein structures (e.g. protein loops)
- Optimization of peptides, e.g. peptide ligands with enhanced binding potency/selectivity and protease stability
Figure 1. One step cleavage and cyclization of unprotected peptides with the KAHA ligation. (Florian, R.; et al. 2015)
Peptide Cyclization Reaction Platform
- Cyclization of solid-phase-synthesized peptides in solutions and on resins
- Cyclization of in vitro translated mRNA-peptide fusion libraries on oligonucleotide (dT) resins
Our Strategy
- Cys-Cys cyclization
- Cys-Cys cyclization is our most commonly applied method of peptide cyclization, where a disulfide (S-S) bond is formed between the thiol side chains of two cysteine residues in the peptide
- We address the problem of dimerization by conducting cyclization at high dilution conditions
- Backbone-cyclization (amide bond formation)
- At BOC Sciences, backbone-amide cyclized peptides are prepared by head-to-tail cyclization or side-chain-to-side chain cyclization
- Our extensive experience and expertise in amidocyclic peptide synthesis strategies can minimize undesired side reactions, such as racemization or peptide capping by coupling reagents
- We are able to provide effective head-to-tail cyclization services using optimal cyclization reagents and cyclization conditions
- Thioether cyclization
What we Deliver?
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
- Our expert teams can provide peptide library containing up to 50 amino acid if there is special demand
- Standard delivery times of around 3-4 weeks depending on different library size and peptide length
- Delivered as freeze-dried in 96-well plates for subsequent experiments
- Analytical MS and HPLC data are available for each peptide product
- BOC Sciences offers standard TFA removal service according to the customer's requirements
BOC Sciences provides professional, rapid and high-quality services of Peptide Cyclization Library design at competitive prices for global customers. Personalized and customized services of Peptide Cyclization Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Florian, R.; et al. Spontaneous head-to-tail cyclization of unprotected linear peptides with the KAHA ligation. Chemical Science. 2015. 6(8): 4889-4896.
