Peptide Pools for Immunopeptidomics

In immunopeptidomics studies, validation of novel epitopes within short non-tryptic peptide sequences still remains challenging due to limited sensitivity and specificity. Moreover, LC-MS/MS measurements of approximately 170,000 synthetic peptides have showed that more than 10% of the spectral data did not correspond to the respective synthetic references with standard data analysis routines, demonstrating the urgent need for the application of reference peptides in immunopeptidomics.

BOC Sciences has the ability to design and manufacture peptide pools in a fast and cost effective way, aiming to strudy the immunopeptidomics issues.

Application

• Mass spectrometry-based new epitopes of prioritization
• Neo-epitope identification
• HLA ligand identification and optimization

Figure 1. Analysis of Endogenous Peptide Pools. (Igor, F.; et al. 2010)

Our Services

• At BOC Sciences, peptide tools for immune monitoring are manufactured using traditional peptide synthesis methods with the addition of measures to avoid contamination that would impede T cell stimulation or independently cause stimulation
• Our well-purified peptides and fully characterized pools provide the reliable assay data required for cell-based immune monitoring
• We also offer custom peptide pool for Immunopeptidomics
• MHC I and MHC II references for binding HLA peptides by mass spectrometry are available
• Our pools for immunopeptidomics can provide fast and inexpensive access to reference peptides
• Our peptide pools have allowed for improved accuracy of neo-epitope identification
• We can deliver high sensitivity to low abundance epitopes as needed
• Light or stable isotope labeled is supported

What we Deliver?

• At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
• LC-MS on marker peptides (up to 8) in final pool to guarantee presence of all peptides within the final pool
• Standard delivery times of around 3-4 weeks depending on different pool size
• Delivered as 96-well plate or 100-tube rack
• CCOA, MS, and HPLC validation data for each peptide before pooling are provided

BOC Sciences provides professional, rapid and high-quality services of Peptide Pools for Immunopeptidomics design at competitive prices for global customers. Personalized and customized services of Peptide Pools for Immunopeptidomics design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!