DEL Oligo Design and Synthesis

Oligonucleotides, or oligos, are short single strands of synthetic DNA or RNA that serve as the starting point for many molecular biology and synthetic biology applications. From genetic examination to forensic research and next-generation sequencing, an oligo plays an important role in these fields as a starting point. The core of the technology of constructing DNA-encoded library is the DNA coding sequence, that is, the codon sequence. The codon sequence records many important information of the building blocks. In addition, the codon sequence can be used in PCR amplification with the application of primer. The DNA coding sequence information can be read by a high-throughput sequencer. Finally, the enriched compounds after screening can be identified. BOC Sciences provides one-stop service of DEL oligo design and synthesis.

Figure 1. DNA-encoded chemistry provides DNA-barcode tagged small molecules that are concatenated to building blocks. (Potowski, M.; et al. 2019)

Our Services

To make it easier and better for researchers, BOC Sciences provides DEL oligo synthesis and DEL oligo optimization services in a competitive fashion. Our rapid and high quality services are as follow:

DEL oligosynthesis

• We provide customized design and synthesis of the codon sequence according to the connection mode and the dimension of the DNA-encoded library
• Our teams have established comprehensive design principles including the consideration of Guanine-cytosine nucleotide content, balance of ATCG bases, palindrome, hairpin, the number of identical consecutive bases and repeat base pairs, etc.
• We ensure that each pair of codon sequence have enough Hamming Distance to distinguish different codons.
• BOC Sciences provides> 50,000 codon sequences with satisfying quality.

DEL oligo optimization

BOC Sciences has created reliable codon optimization algorithm to optimize key parameters to stabilize DNA sequences and improve gene expression. Our well-designed algorithm is continuously updated and improved based on empirical data to address the following parameters:

• Codon usage bias
• GC nucleotide content
• mRNA secondary structure and unstable motifs
• Repeat sequences (direct repeat, inverted repeat, and dyad repeat)
• Restriction enzyme recognition sites
• Custom modifications (e.g. ribosome binding site modifications and RNA motif alterations)

BOC Sciences provides professional, rapid and high-quality services of DEL oligo design and synthesis at competitive prices for global customers. Personalized and customized services of DEL oligo design and synthesis can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!