Scrambled Library
A scrambled library consists of all alternative sequences of a given peptide, which means that all amino acid residues of the initial sequence are mixed to form all possible combinations of amino acids.
BOC Sciences uses the perturbation library to identify peptides with specific functions or activities in a set of active sequences.
Applications
- Sequence optimization
- Serves as a negative control to find a particular sequence that is critical for stability or bioactivity of a protein
- Target protein screening: find new leads by creating random screening libraries
- Used as a targeting molecular probe for proteins, antibodies, genes, etc
Figure 1. Third peptide contains the scrambled sequence MLLVTPSG as a control. (Bahney, C. S.; et al. 2011)
Library Design
- Our scrambled library is constructed based on the internal substitution of the amino acid sequence of an original peptide
- The number of each amino acid (e.g. 2 arginines) in the peptide is the same, but its position with the rest of the amino acids is random
- We can deliver a maximum of 1,000 permutations of your original sequence
- Our expert teams can also provide additional services of designing larger sets of library of all3-mer or 4-mer peptides containing every possible combination and permutaion of amino acid
- The most mutated peptide libraries, showing all possible peptide substitution structures
What we Deliver?
- At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
- Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
- Our expert teams can provide peptide library containing up to 50 amino acid if there is special demand
- Standard delivery times of around 3-4 weeks depending on different library size and peptide length
- Delivered as freeze-dried in 96-well plates for subsequent experiments
- MS and HPLC analysis results are provided for each peptide product
- All peptides in the library can be de-TFA salt treatment, also can be converted to acetate according to the customer's requirements
BOC Sciences provides professional, rapid and high-quality services of Scrambled Library design at competitive prices for global customers. Personalized and customized services of Scrambled Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Bahney, C. S.; et al. A bioresponsive hydrogel tuned to chondrogenesis of human mesenchymal stem cells. The FASEB Journal. 2011. 25(5).
