Alanine Scanning Peptide Library

Aiming to accurately identify residues that play a role in peptide activity, scientific experts have employed many strategies to design and synthesize alanine scanning libraries. Alanine can be used to replace non-alanine residues of the original peptide. The measurement of the corresponding change in epitope activity can reflect the contribution of each residue to the function of a given protein, helping to identify the specific amino acid residues that are closely related to peptide function, stability and conformation.

Advantages of Using Alanine

• Small size
• Chemical inertness
• Methyl functionality

Applications

• Explore the effect of single amino acid side chains on protein function and properties
• Study protein structure-function relationships
• Identify binding sites and key epitope motifs
• Design more stable proteins or proteins with altered properties
• Evaluate functionally active groups of enzymes

Figure 1. Systematic alanine scanning. (Johnston, H. J.; et al. 2016)

Alanine Scanning Mutagenesis Technology

BOC Sciences has introduced this powerful tool to construct our unique libraries to identify B-cell epitopes.

• Mutagenesis

Firstly, each non-alanine residue of the peptide will be substituted sequentially by one alanine at a time by employing a a highly specified site-directed mutagenesis approach

• Expression

Then, a large number of plasmid clones will be expressed accurately and rapidly

• Detection

Finally, we measure the corresponding changes in peptide-antibody binding activity

What we Deliver

• At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
• Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
• Our expert teams can also provide peptide library containing up to 50 amino acid
• Delivery times of around 3-4 weeks
• Support the delivery of freeze-dried peptides in 96-well plates for subsequent experiments
• MS and HPLC analysis results are provided for each peptide product
• Delivered as TFA-salts as lyophilized powder

BOC Sciences provides professional, rapid and high-quality services of Alanine Scanning Peptide Library design at competitive prices for global customers. Personalized and customized services of Alanine Scanning Peptide Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!