Alanine Scanning Peptide Library

Aiming to accurately identify residues that play a role in peptide activity, scientific experts have employed many strategies to design and synthesize alanine scanning libraries. Alanine can be used to replace non-alanine residues of the original peptide. The measurement of the corresponding change in epitope activity can reflect the contribution of each residue to the function of a given protein, helping to identify the specific amino acid residues that are closely related to peptide function, stability and conformation.

Advantages of Using Alanine

Small size
Chemical inertness
Methyl functionality

Applications

Explore the effect of single amino acid side chains on protein function and properties
Study protein structure-function relationships
Identify binding sites and key epitope motifs
Design more stable proteins or proteins with altered properties
Evaluate functionally active groups of enzymes

Figure 1. Systematic alanine scanning. (Johnston, H. J.; et al. 2016)

Alanine Scanning Mutagenesis Technology

BOC Sciences has introduced this powerful tool to construct our unique libraries to identify B-cell epitopes.

Mutagenesis

Firstly, each non-alanine residue of the peptide will be substituted sequentially by one alanine at a time by employing a a highly specified site-directed mutagenesis approach

Expression

Then, a large number of plasmid clones will be expressed accurately and rapidly

Detection

Finally, we measure the corresponding changes in peptide-antibody binding activity

What we Deliver

At BOC Sciences, peptide arrays are provided in a variety of sizes and formats according to the demand of different projects
Peptide lengths can be ranging from 5 to 30 amino acids, which can meet most requirement of applications
Our expert teams can also provide peptide library containing up to 50 amino acid
Delivery times of around 3-4 weeks
Support the delivery of freeze-dried peptides in 96-well plates for subsequent experiments
MS and HPLC analysis results are provided for each peptide product
Delivered as TFA-salts as lyophilized powder

BOC Sciences provides professional, rapid and high-quality services of Alanine Scanning Peptide Library design at competitive prices for global customers. Personalized and customized services of Alanine Scanning Peptide Library design can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!

Reference

Johnston, H. J.; et al. Naturally Inspired Peptide Leads: Alanine Scanning Reveals an Actin-Targeting Thiazole Analogue of Bisebromoamide. ChemBioChem. 2016. 17(17): 1621-1627.

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Services Based on the Chemical Library Design Platform

BOC Sciences has rich experience in working with global customers in custom library synthesis of compounds and generating small to medium-sized libraries of target compounds. Our knowledge in generating a large number of target molecules in a remarkably shorter time enables quick biological screenings for affinities. With the target properties in mind, we deliver target molecules, by applying our extensive knowledge in drug discovery.

Our mission is to provide clients with a professional chemical library design platform. Empowered by high-quality services and effective research solutions, we are committed to helping customers achieve effective and successful research goals.