Introduction of Primer
A primer is synthesized by stimulation at the beginning of nucleotide polymerization. A primer is a macromolecule with a specific nucleotide sequence, which is connected to the reactant in the form of hydrogen bonds. Primers are usually two artificially synthesized oligonucleotide sequences. One primer is complementary to a DNA template strand at one end of the target region, and the other primer is complementary to another DNA template strand at the other end of the target region. The function of the primer is to act as the starting point of nucleotide polymerization, and the nucleic acid polymerase can synthesize a new nucleic acid strand from its 3 ends. The primers artificially designed in vitro are widely used in polymerase chain reaction, sequencing and probe synthesis, etc.
DEL Primer Design
The purpose of PCR primer design is to find a pair of suitable nucleotide fragments that can effectively amplify the template DNA sequence. In polymerase chain reaction (PCR) technology, the nucleotide sequence of a target gene is known, primers are synthesized based on this sequence, and PCR amplification technology is used to melt the target gene DNA into a single strand after heat denaturation. The single-stranded corresponding complementary sequences are combined, and then extended under the action of a high-temperature DNA polymerase. The cycle is repeated, and the product obtained after the extension can also be combined with the primer.
There are 3 basic principles for DEL primer design:
- First, the sequence of the primer and the template must be closely complementary
- Second, the formation of a stable dimer or hairpin structure between the primer and the primer should be avoided
- Lastly, the primers cannot initiate DNA polymerization (ie mismatches) at non-target sites of the template
The design of primers needs to consider many factors such as primer length, product length, sequence Tm value (melting temperature), internal stability of the double-stranded primer and template (∆G), the energy value of primer dimer and hairpin formation, the priming efficiency at the false priming site, the GC content of the primer and product ( composition), etc. If necessary, primers need to be modified, such as adding restriction endonuclease sites, introducing mutations, etc.
Figure 1. Synthesis of the DEL by a two stage synthesis strategy. (Klika Škopić, M.; et al. 2016)
Our Services
We provide DEL primers with excellent quality to help you successfully conduct the library construction, and strictly control the factors that may affect your DNA-encoded library construction. We are committed to breaking the barrier between chemical synthesis primers and biological applications. BOC Sciences has developed a complete quality control system based on the process and principles of DNA-encoded library building.
Precise Quantification
Our DEL primers design and synthesis make full use of each single-stranded DNA, so that every pair of positive and negative sense strands are perfectly matched.
- Self-developed quantitative calculation formula for primers
- Disposable items
- Fully automatic quantitative dispensing workstation
High-efficiency Enzyme Linkage Efficiency
At BOC Sciences, we synthesize DEL primers to help prepare primer materials for high-efficiency library construction, ensuring that the primers used in the library construction for enzyme ligation reaction have high ligation efficiency.
- Stability of each primer connection: >95%
- After the primer is dissolved, the pH value is consistent with the requirements of the reaction system: the pH value of the primer after dissolution is maintained between 6-8
Nearly Zero Base Error Rate
Our experts are committed to using DEL primers to create a library-building primer material with zero base error rate to further ensure the precise positioning of the lead compounds selected.
- High-precision MS analysis report for each primer
Professional project management team
- Our team is committed to fully satisfying the requirements for various quality indicators of primers in the process of DNA-encoded library construction
- We have created a professional project management team. Our senior scientists track and manage your DEL primer orders throughout the process
- BOC Sciences has developed a personalized inspection system for your quality inspection standards, saving you a lot of time and labor costs incurred in the quality inspection of raw materials before the library building experiment
BOC Sciences provides professional, rapid and high-quality services of DEL primer design and synthesis at competitive prices for global customers. Personalized and customized services of DEL primer design and synthesis can satisfy any innovative scientific study demands. Our clients have direct access to our staff and prompt feedback to their inquiries. If you are interested in our services, please contact us immediately!
Reference
- Klika Škopić, M.; et al. Design and synthesis of DNA-encoded libraries based on a benzodiazepine and a pyrazolopyrimidine scaffold. Med. Chem. Comm. 2016, 7: 1957-1965.
